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BRAIN SLICES I PAIRED RECORDINGS I PRESYNAPTIC CALCIUM IMAGING I OPTOGENETICS I DYNAMIC CLAMP I VIRAL EXPRESSION OF PROTEINS I IN VIVO RECORDINGS I IMMUNO I SERIAL E.M.

IMMUNOHISTOCHEMICAL TECHNIQUES                                                                                                Immunohistochemical techniques are used to determine the location of proteins. In this example a calcium dependent isoform of PKC (green) is shown to be present at the calyx of Held.  In these images the red fluorescence shows vGlut1, which is used to identify calyces of Held, which surround the cell boldies of neurons in the MNTB region (spheres that lacking fluoresence).

WADE REGEHR                                                          DEPARTMENT OF NEUROBIOLOGY                                     HARVARD MEDICAL SCHOOL